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Comparative determination of glycated hemoglobin with direct photometric immunoassay and affi nity chromatography

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dc.contributor.author Vucheva, E.
dc.contributor.author Genova, М.
dc.contributor.author Niagolov, Y.
dc.contributor.author Doncheva, N.
dc.contributor.author Peichinova, G.
dc.contributor.author Tsatchev, K.
dc.date.accessioned 2014-07-10T09:32:39Z
dc.date.available 2014-07-10T09:32:39Z
dc.date.issued 2012-06
dc.identifier.citation E. Vucheva, М. Genova, Y. Niagolov, N. Doncheva, G. Peichinova and K. Tsatchev. Comparative determination of glycated hemoglobin with direct photometric immunoassay and affi nity chromatography - Acta medica bulgarica , 39, 2012, No 2, 3-10. en_US
dc.identifier.issn 0324-1750
dc.identifier.uri http://hdl.handle.net/10861/542
dc.description Address for correspondence: Assoc. Prof. Nadezhda Doncheva, MD, PhD; Medical Institute − Ministry of Interior; Department of Clinical Laboratory, 79 A, Skobelev str., 1606 Sofia, Bulgaria. tel: +3592 9821-416 ; e-mail: ndon4eva@abv.bg en_US
dc.description.abstract Glycated hemoglobin (HbA1c) is the gold standard in long-term assessment of glycaemic control in patients with diabetes mellitus (DM). Liquid Chromatography-Isotope Dilution-Mass Spectrometry is a reference method for HbA1c, but other methods used in practice need standardization and comparison. This study was aimed to perform a comparative evaluation of the results from HbA1c determination by direct immuno-photometric method (A) and affi nity chromatography method (B). The study included 50 samples with values from all clinically measurable areas. HbA1c in whole blood was studied using Olympus AU 400 analyzer and NycoCard READER II by, applying method A and method B, respectiovely. The results are compared using the program Method Validator, as for the reference method the chromatographic method was accepted. The imprecision in series for method A has been traced in 10 parallel samples from two patients in border and abnormal area of values. The inaccuracy was evaluated in normal and pathological human control material for 25 consecutive days. The results from correlation and regression analysis show linear regression coeffi cient r = 0.949, slope = 0.919 and intercept = 0.59. Assessment of imprecision for method A shows CV values of 1.21% and 1.91% for both cases, respectively. Accuracy monitoring of method A with control materials shows d% 4.979 in the normal area, and 5.033 in the pathological area. The results show high positive correlation and comparability between the two methods. Direct immuno-photometric method has good accuracy and precision and is applicable for analyzer Olympus AU 400. en_US
dc.language.iso en en_US
dc.publisher Централна медицинска библиотека, МУ София / Central Medical Library - MU Sofia en_US
dc.subject Glycated haemoglobin, direct immuno-photometric method, affi nity chromatography method, NycoCard READER en_US
dc.title Comparative determination of glycated hemoglobin with direct photometric immunoassay and affi nity chromatography en_US
dc.type Article en_US


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  • Acta Medica Bulgarica
    Многопрофилно списание, излизащо само на английски език. Съдържа авторски статии от водещи български медицински специалисти, отразяващи върховите постижения на българската медицина наука и практика. Публикуват се материали, отразяващи резултати от експериментални проучвания, клиника и казуистика. Излиза 2 пъти годишно. ISSN:0324-1750

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