Central Medical Library - Sofia

Methods of protein delivery into mammalian cells for gene therapy and genetic studies

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dc.contributor.author Todorova, R.
dc.date.accessioned 2012-05-04T12:44:28Z
dc.date.available 2012-05-04T12:44:28Z
dc.date.issued 2008-06
dc.identifier.citation Todorova, R. Methods of protein delivery into mammalian cells for gene therapy and genetic studies - Acta Medica Bulgarica, 35, 2008, № 1, 3-11. en_US
dc.identifier.issn 0324-1750
dc.identifier.uri http://hdl.handle.net/10861/143
dc.description Address for correspondence: R. Todorova Institute of Biophysics Bulgarian Academy of Sciences 1113 Sofia Bulgaria 02 979-31-25 e-mail: todorova@obzor.bio21.bas.bg en_US
dc.description.abstract Methods, lying on different principles for introducing proteins into mammalian cells were compared in the present study. Some of them rely on non-covalent complex formation (Chariot), osmotic lysisis of pinocytic vesicles (Influx pinocytic cell-loading reagent), electric power (electroporation), lipid-based delivery system (Bioporter), microinjection, small protein transduction domains (PTDs) from viral proteins, bacterial secretion system type III (T3SS). Thus, the delivery by many of the compared methods can give a sufficient number of uniformly loaded cells for different studies including transcription and translation, cell cycle regulation, control of apoptosis, oncogenesis and gene therapy. en_US
dc.language.iso en en_US
dc.publisher Централна медицинска библиотека, МУ София / Central Medical Library - MU Sofia en_US
dc.subject protein delivery, mammalian cells, gene therapy, electroporation, bio-porter, microinjection, nanoparticles, cell-penetrating peptides, pinocytic vesicles en_US
dc.title Methods of protein delivery into mammalian cells for gene therapy and genetic studies en_US
dc.type Article en_US


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  • Acta Medica Bulgarica
    Многопрофилно списание, излизащо само на английски език. Съдържа авторски статии от водещи български медицински специалисти, отразяващи върховите постижения на българската медицина наука и практика. Публикуват се материали, отразяващи резултати от експериментални проучвания, клиника и казуистика. Излиза 2 пъти годишно. ISSN:0324-1750

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